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Ionization and fragmentation by keV ions and energetic photons of protonated oligonucleotides produced by Electrospray Ionization

Tiemens, M. (2012) Ionization and fragmentation by keV ions and energetic photons of protonated oligonucleotides produced by Electrospray Ionization. Master's Thesis / Essay, Physics.

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Abstract

The effects of different types of ionising radiation on gas phase oligonucleotides d(GCAT) and d(GTAT) has been investigated by irradiation with keV ions and VUV and soft X-ray photons (15 – 570 eV) using time-of-flight mass spectroscopy. The molecules were brought into the gas phase using Electrospray Ionisation. On all instances, the molecules showed similar dissociation patterns, mostly involving both charged and protonated nucleobase loss and the formation of a specific fragment of 2-deoxy-D-ribose. By also taking into account less pronounced product fragments, information on the weak spots of the molecules could be deduced. Hierarchies for the nucleobase yields were constructed, and two models have been proposed to explain the trends observed. Unlike in experiments performed on single deoxyribose molecules, the readily observed fragments showed that a significant part of these molecules is able to survive irradiation when they are incorporated into larger structures.

Item Type: Thesis (Master's Thesis / Essay)
Degree programme: Physics
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 15 Feb 2018 07:49
Last Modified: 15 Feb 2018 07:49
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/10341

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