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Using single-cell rna sequencing to study host-pathogen interactions

Mestre-Farràs, N (2016) Using single-cell rna sequencing to study host-pathogen interactions. Master's Thesis / Essay, Molecular Biology and Biotechnology.

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Abstract

Populations of cells are heterogeneous. Despite single cells of a population present common gene expression regulation they also present small differences in the expression. Single cell methodologies are needed to study the impact of this heterogeneity in cellular processes. Single-cell RNA sequencing (scRNAseq) has already been used to study heterogeneity of eukaryotic cell populations at transcriptional level. The aim of this report is to review the current state of scRNAseq technology to apply it for the study of host-pathogen interactions. Studies that already used this technique to study immune cells response to lipopolysaccharides showed different activation thresholds between host cells under same stimuli and that heterogeneity in pathogenic cells influences heterogeneity in host cells response. Therefore, scRNAseq is a powerful tool to characterize expression heterogeneity during host-pathogen interactions.

Item Type: Thesis (Master's Thesis / Essay)
Degree programme: Molecular Biology and Biotechnology
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 15 Feb 2018 08:11
Last Modified: 15 Feb 2018 08:11
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/13740

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