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Dynamics within the Sec translocon: Binding of Ribosome Nascent Chain complexes to SecYEG

Koning, M. de (2016) Dynamics within the Sec translocon: Binding of Ribosome Nascent Chain complexes to SecYEG. Research Project 1 (minor thesis), Molecular Biology and Biotechnology.

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Abstract

SecYEG is one of the main components of the Sec-translocon in Escherichia coli. Via two targeting routes, this system is responsible for translocating proteins across or incorporate proteins into the cytoplasmic membrane. The latter is done through the association of a ribosome nascent chain complex to the SecYEG channel. To gain more insight, both SecYEG and the RNCs were purified from E. coli expression strains. SecYEG was reconstituted into proteoliposomes and nanodiscs to test its translocation activity and the binding ability to RNCs. Through these experiments a KD in the nanomolar range was determined for the binding of RNCs to SecYEG nanodiscs.

Item Type: Thesis (Research Project 1 (minor thesis))
Degree programme: Molecular Biology and Biotechnology
Thesis type: Research Project 1 (minor thesis)
Language: English
Date Deposited: 15 Feb 2018 08:25
Last Modified: 15 Feb 2018 08:25
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/14642

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