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Tools and Applicatiosn of CRISPR-Cas in prokaryotes and eukaryotes

Kuijpers, L.J. (2017) Tools and Applicatiosn of CRISPR-Cas in prokaryotes and eukaryotes. Master's Thesis / Essay, Molecular Biology and Biotechnology.

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Abstract

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems are an acquired and adaptive immune system of archaea and bacteria. By using a small guide RNA (sgRNA) and a single protein or a cascade of proteins they are able to target and degrade exogenous DNA or RNA. This bacterial defence system since has been studied extensively, which has lead to several key discoveries enabling its use as a programmable molecular tool. CRISPR-Cas systems allow scientists to perform insertions, deletions and translocations with high target specificity. With modified Cas proteins, several more tools have been developed that allow control of gene expression or visualization of chromosomes. In this review the possible tools and applications of CRISPR-Cas in both prokaryotes and eukaryotes are shown and discussed.

Item Type: Thesis (Master's Thesis / Essay)
Degree programme: Molecular Biology and Biotechnology
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 15 Feb 2018 08:26
Last Modified: 15 Feb 2018 08:26
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/14902

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