Javascript must be enabled for the correct page display

Identification and Production Of Novel Lantibiotics From Clostridium Species Via Heterologous Expression in Lactococcus lactis

Jati, Afif Pranaya (2018) Identification and Production Of Novel Lantibiotics From Clostridium Species Via Heterologous Expression in Lactococcus lactis. Research Project 1, Molecular Biology and Biotechnology.

[img]
Preview
Text
mMBB_2018_APJati_Research Project I.pdf

Download (2MB) | Preview
[img] Text
toestemming.pdf
Restricted to Registered users only

Download (96kB)

Abstract

The capability of nisin induced gene expression system (NICE) in Lactococcus lactis to modify small peptides into lanthipeptides, provide an exciting opportunity for novel antimicrobials production to combat various pathogens. This system can be exploited to produce “new to nature” lantibiotics from various organisms using synthetic biology approach. This study aimed to identify and produce novel lantibiotics from the genus of Clostridium that had not been discovered. As the result, 54 putative clostridia new lantibiotic genes were discovered after in silico analysis with BAGEL3 and Anti-SMASH. Subsequently, based on the novelty and the compatibility to expression system, twelve putative clostridia lantibiotic genes were selected as candidates and tested for production via heterologous expression in Lactococcus lactis. Interestingly, eleven peptides except Clos16 displayed antimicrobial activity against L. lactis NZ9000 as indicator strain. Moreover, Micrococcus flavus is susceptible to Clos4, Clos12, Clos22 and ClosDP. Additionally, Clos2, Clos14 and ClosDP lantibiotics showed antimicrobial activity against Clostridium sporogenes and Micrococcus luteus. Nevertheless, mass spectrometry analyses using MALDI-TOF and LC-MS/MS revealed multiple dehydrated serines and/or threonines in several putative lantibiotic candidates, confirming successful production and secretion of novel lantibiotics from the genus of Clostridium by nisin synthetic machinery.

Item Type: Thesis (Research Project 1)
Supervisor:
Supervisor nameSupervisor E mail
Kuipers, O.P.O.P.Kuipers@rug.nl
Degree programme: Molecular Biology and Biotechnology
Thesis type: Research Project 1
Language: English
Date Deposited: 20 Feb 2018
Last Modified: 27 Mar 2018 13:33
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/16492

Actions (login required)

View Item View Item