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Effect of heat treatment on the density of Wolbachia and the remaining microbiome in the host Asobara japonica.

Basu, Meghna (2018) Effect of heat treatment on the density of Wolbachia and the remaining microbiome in the host Asobara japonica. Research Project 1, Biomedical Sciences.

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Abstract

Most of the symbionts that are present within the microbiome of a host contribute towards the host fitness by modifying the host’s phenotype. However, the role of individual symbiont remains ambiguous. In this study we attempt to eliminate one endosymbiont Wolbachia from the microbiome of the host Asobara japonica using heat treatment. After the application of heat shock at 38°C for 15 minutes, the effect on the Wolbachia titre and the microbiome of the host were analyzed using qPCR and DGGE. The results indicated that heat shock had a direct impact on the Wolbachia titre of the host and led to a lower concentration in the progeny of the heat shocked mothers. DGGE analysis showed that the heat treatment did not have any significant effect on the diversity of the microbiome. We suggest that heat can be used as a treatment condition to study changes in the microbiome caused by lowering of the Wolbachia titre.

Item Type: Thesis (Research Project 1)
Supervisor:
Supervisor nameSupervisor E mail
Kuipers, O.P.O.P.Kuipers@rug.nl
Beukeboom, L.W.L.W.Beukeboom@rug.nl
Degree programme: Biomedical Sciences
Thesis type: Research Project 1
Language: English
Date Deposited: 09 Apr 2018
Last Modified: 16 Apr 2018 09:49
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/16668

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