Kappert, Koen (2019) Evaluation of a gene editing system suitable for the evaluation of the effect of hat and hdac inhibitors on the efficiency of the crispr-cas9 technique. Bachelor's Project, Pharmacy.
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Abstract
In this report an experiment is described that evaluates the use of a gene editing system suitable for the evaluation of the effect of HAT and HDAC inhibitors on the efficiency of the CRISPR-Cas9 technique. The system consists of H27 cells, which are HeLa cells that stably express eGFP gene. It is desired that the eGFP gene is knocked-out and the eYFP gene from a donor plasmid is knocked in with homology directed repair. The knock-in efficiency is measured with plate reader and FACS, respectively. It is concluded that no knock-in takes place, but knock-out does take place. The system is insufficient for the evaluation of the effect of HAT and HDAC inhibitors on the efficiency of the CRISPR-Cas9 technique. It is recommended that a different system is used.
| Item Type: | Thesis (Bachelor's Project) |
|---|---|
| Supervisor name: | Haisma, H.J. |
| Degree programme: | Pharmacy |
| Thesis type: | Bachelor's Project |
| Language: | English |
| Date Deposited: | 04 Apr 2019 |
| Last Modified: | 04 Apr 2019 12:30 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/19331 |
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