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Evaluation of a gene editing system suitable for the evaluation of the effect of hat and hdac inhibitors on the efficiency of the crispr-cas9 technique

Kappert, Koen (2019) Evaluation of a gene editing system suitable for the evaluation of the effect of hat and hdac inhibitors on the efficiency of the crispr-cas9 technique. Bachelor's Project, Pharmacy.

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Abstract

In this report an experiment is described that evaluates the use of a gene editing system suitable for the evaluation of the effect of HAT and HDAC inhibitors on the efficiency of the CRISPR-Cas9 technique. The system consists of H27 cells, which are HeLa cells that stably express eGFP gene. It is desired that the eGFP gene is knocked-out and the eYFP gene from a donor plasmid is knocked in with homology directed repair. The knock-in efficiency is measured with plate reader and FACS, respectively. It is concluded that no knock-in takes place, but knock-out does take place. The system is insufficient for the evaluation of the effect of HAT and HDAC inhibitors on the efficiency of the CRISPR-Cas9 technique. It is recommended that a different system is used.

Item Type: Thesis (Bachelor's Project)
Supervisor:
Supervisor nameSupervisor E mail
Haisma, H.J.H.J.Haisma@rug.nl
Degree programme: Pharmacy
Thesis type: Bachelor's Project
Language: English
Date Deposited: 04 Apr 2019
Last Modified: 04 Apr 2019 12:30
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/19331

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