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RNA in bacterial extracellular vesicles

de Vries, Sjoerd Jan (2019) RNA in bacterial extracellular vesicles. Colloquium, Molecular Biology and Biotechnology.

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Abstract

Extracellular vesicles or outer membrane vesicles (OMVs) are produced by all domains of life and can contain biomolecules, such as DNA, RNA, and proteins. They are 20-1000nm in size, depending on the producer cell. In this colloquium, we will look at the RNAs in OMVs produced by gram-negative bacteria. Because this is a new and emerging field, the research papers are scattered. In 2014, proof of RNA in OMVs was published. It was found that E. coli produces OMVs, containing mostly non-coding RNAs smaller than 60 nt. A large part of these RNAs are tRNA fragments. A large amount of possible human target genes was determined from OMV miRNA from periodontal pathogens. These miRNAs were 16-25bp in size and were able to decrease cytokine expression in Jurkat T cells. In P. aeruginosa, similar sRNAs were found, including fragments from tRNA-Pro, tRNA-Met, and tRNA-Arg. The fragment of tRNA-Met was used to test if OMV sRNAs are transferred to host cells. It was found that the fragment could be detected in HBE cells, and could suppress an immune response in cell lines. These findings suggest that OMVs are of great importance in pathogeny, where RNAs play a major role.

Item Type: Thesis (Colloquium)
Supervisor:
Supervisor nameSupervisor E mail
Billerbeck, S.K.S.K.Billerbeck@rug.nl
Degree programme: Molecular Biology and Biotechnology
Thesis type: Colloquium
Language: English
Date Deposited: 24 Sep 2019
Last Modified: 25 Sep 2019 09:27
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/21020

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