Validating the Poeciliopsis animal model: Effects of perinatal fluoxetine exposure on mother and offspring of live bearing fish with and without placentation

Haan, F (2020) Validating the Poeciliopsis animal model: Effects of perinatal fluoxetine exposure on mother and offspring of live bearing fish with and without placentation. Master's Research Project 1, Biomedical Sciences.

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Abstract

Serotonin plays a vital role in the early development across vertebrates. Alterations in the maternal serotonergic system may have deleterious effects for the developing embryo. The placenta is an active maternal-foetal intermediary and perturbations of the maternal milieu can be transmitted to the foetus through the placenta. To gain more insight in the mediating effects of the placenta during pregnancy, we investigated the impact of altered maternal serotonin levels in two species of live-bearing fish, Poeciliopsis gracilis without a placenta and Poeciliopsis turneri with a placenta. Both species mothers were treated with a selective serotonin reuptake inhibitor fluoxetine in concentrations ranging from environmental (0 µg/L, 0.1 µg/L, 0.5 µg/L, 1 µg/L) to human treatment levels (10 µg/L, 20 µg/L, 40 µg/L) for six weeks after a prior gestation period of 28 days. During the six weeks of chronic fluoxetine administration offspring were collected daily. It was observed that the weights of both species’ mothers were not affected by the fluoxetine treatment. P. gracilis offspring length was changed in an inverted-u shape where the middle range of fluoxetine concentration resulted in longer offspring than those exposed to the lowest and highest tested concentrations. P. turneri offspring was observed to be affected by fluoxetine in a dose-responsive matter where more fluoxetine resulted in a smaller offspring length. P. gracilis offspring were found to be heavier regardless of t

Item Type:	Thesis (Master's Research Project 1)
Supervisor name:	Olivier, J.D.A. and Staal, L.
Degree programme:	Biomedical Sciences
Thesis type:	Master's Research Project 1
Language:	English
Date Deposited:	14 Apr 2020 10:52
Last Modified:	14 Apr 2020 10:52
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/21748

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