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Validation of the semen analysis method used by the University Medical Center Groningen

Kuntzel, Leonie (2020) Validation of the semen analysis method used by the University Medical Center Groningen. Master's Research Project 2, Biomedical Sciences.

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Abstract

The current computer-assisted semen analysis method used by the UMCG is not always reliable and prone to technical errors, this project focusses on validating a new manual counting method to determine sperm concentration. Therefore the exact number of counted spermatozoa per field of view was counted to determine the concentration, via a calculation method that converts a number of counted spermatozoa per field into a number of spermatozoa per ml. It was determined how many fields of view per sample have to be counted in order to reach a reliable estimation of the concentration (<25% deviation of the real concentration), distinguishing between used objective (20X/40X) and location in a counting chamber. A separate part of the project was data analysis of a database containing data about semen motility parameters and fertility outcomes (fertilized oocytes/ achieved pregnancy), in an attempt to discover a potential correlation between the parameters and fertility outcome. This data analysis was performed in R studio.

Item Type: Thesis (Master's Research Project 2)
Supervisor name: Hoek, A.
Degree programme: Biomedical Sciences
Thesis type: Master's Research Project 2
Language: English
Date Deposited: 24 Aug 2020 13:38
Last Modified: 24 Aug 2020 13:38
URI: https://fse.studenttheses.ub.rug.nl/id/eprint/23177

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