Cornelissen, Julian, J.B.C. (2022) Designing an enzyme with mono-oxygenase activity and a minimal scaffold that can be immobilized onto a glassy carbon electrode. Bachelor's Project, Pharmacy.
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Abstract
To study metabolites that are being made from drugs in the body, a novel electrochemical system will be designed. A new enzyme called FeMC7 will be engineered for this system. The enzyme will have a cysteine bound to the iron, and the amino acids serine and aspartate will be present in the scaffold nearby the iron on the distal side. These features will assure that the enzyme will behave as a native cytochrome P450 enzyme and have mono-oxygenase activity. The enzyme will be immobilized on a glassy carbon electrode. The terminal end of the D and TD chain will be used in a carbodiimide reaction to immobilize the enzyme.
| Item Type: | Thesis (Bachelor's Project) |
|---|---|
| Supervisor name: | Le, P.H.M. |
| Degree programme: | Pharmacy |
| Thesis type: | Bachelor's Project |
| Language: | English |
| Date Deposited: | 11 Apr 2022 12:46 |
| Last Modified: | 11 Apr 2022 12:46 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/26865 |
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