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The significance of glutathione in protection of Brassica pekineAsis against hydrogen peroxide and paraquat toxicity

Koppenaal, E.C. (2007) The significance of glutathione in protection of Brassica pekineAsis against hydrogen peroxide and paraquat toxicity. Master's Thesis / Essay, Biology.

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Abstract

In oxidative stress there are many antioxidants which protect the plant, glutathione is one of them. Hydrogen peroxide and paraquat (for its generation of superoxide) are two compounds that cause stress in plants and are used in many researches about stress and the resistance of plants against it. In this research the question was searched whether glutathione does protect when the plant is under stress caused by hydrogen peroxide or paraquat and decreased enzyme activity. The conductivity for paraquat and also the fluorescence showed a increase in damage in both temperature treatments but especially the cold (low enzyme activity) treatment. The pigments showed a similar result only the warm leaf discs were bleached and the cold leaf discs had a decrease but still they were green. The thiol-content decreased also more at the warm temperature, and not as much when chilled. For hydrogen peroxide the results for pigment and thiols were the same as the ones for paraquat. There is a decrease, but it is only very slightly in the cold, and more in the warmth. The decrease only happened at the very high concentration of 512 mM H202. The conductivity showed the same results as with paraquat, but the fluorescence of hydrogen peroxide was in accordance wit the pigments and thiol-content. Again it only happened at the highest concentration. There is no indication that glutathione protects, and that the tests using hydrogen peroxide and paraquat are very trustworthy.

Item Type: Thesis (Master's Thesis / Essay)
Degree programme: Biology
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 15 Feb 2018 07:31
Last Modified: 15 Feb 2018 07:31
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/9107

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