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Barriers in the generation of iPS cells

Overkamp, J. (2010) Barriers in the generation of iPS cells. Bachelor's Thesis, Biology.

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Abstract

Since the discovery of induced pluripotent stem (iPS) cells by Takahasi and Tamanaka in 2006, a new era in regenerative medicine has began. Takahasi and Tamanaka were able to reprogram somatic cells back to pluripotent state by introducing retroviral the pluripotency genes Oct4, Sox2, Klf4 and c-Myc. After minor refinements in the initial reprogramming protocol, the iPS cells were shown to be germline-competent and capable of forming any cell type. This technology could deliver patient-specific derived iPS cells that can contribute to disease modeling, drug screening, toxicology tests and autologous cell-based therapies. However, there are still limits that indicate that iPS cell technology is still at its infancy. The reprogramming efficiency of iPS cells is very low and generated iPS cells are highly oncogenic. 4 years since the discovery of iPS cells, plenty of research has been done concerning iPS cells. New methods have been developed to refine and improve reprogramming and factors have been tested to improve reprogramming efficiency. This review will focus on why the generation of iPS cells is so inefficient and how the most recent developed methods can improve it. I will discuss the basic parameters of somatic cell reprogramming; why iPS cell generation is a long and inefficient process; newly developed methods to generate iPS cells; and factors that can improve reprogramming efficiency. With these topics I will try to answer the following question: what is the most efficient way to generate iPS cells?

Item Type: Thesis (Bachelor's Thesis)
Degree programme: Biology
Thesis type: Bachelor's Thesis
Language: English
Date Deposited: 15 Feb 2018 07:44
Last Modified: 15 Feb 2018 07:44
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/9325

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