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Discovery of putative monooxygenases from Rhodococcus sp. RHA1

Verhoeven, M. (2009) Discovery of putative monooxygenases from Rhodococcus sp. RHA1. Bachelor's Thesis, Chemistry.

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Abstract

The Baeyer-Villiger reaction inserts an oxygen atom into specific carbon-carbon bonds. The reaction is able to form compounds which form the basis for industrially interesting products. Baeyer-Villiger monooxygenases (BVMOs) are enzymes able to catalyze this specific reaction, and several other oxidation reactions (e.g. sulfoxidation). The BVMOs provide a more attractive alternative to traditional chemistry for performing the reaction. To broaden the range of recognized substrates by available BVMOs, the genome of Rhodococcus jostii RHA1 was screened with two sequences of genes with known function. The cloning of four genes with good (>40%) sequence similarity resulted in the expression and purification of a LimB homolog. The enzyme was found to catalyze sulfoxidation reactions with high enanthioselectivity.

Item Type: Thesis (Bachelor's Thesis)
Degree programme: Chemistry
Thesis type: Bachelor's Thesis
Language: English
Date Deposited: 15 Feb 2018 07:45
Last Modified: 15 Feb 2018 07:45
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/9448

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