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Dynamic FRET: an avenue towards understanding biological macromolecules in motion

Overgaauw, M.S. (2010) Dynamic FRET: an avenue towards understanding biological macromolecules in motion. Bachelor's Thesis, Biology.

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Abstract

Dynamic FRET is a tool within the field of spectroscopy to study the dynamic behavior of biological macromolecules, particularly proteins. In a typical experiment, a protein is covalently labeled with a fluorescent donor and an acceptor at appropriate sites. By comparing the fluorescence intensity decay of the donor alone with that of the donor in presence of the acceptor, one could calculate the distance (nm) between both probes. If the distance is not well defined, a distance distribution appears. The data presented in this thesis is based on a study of the dynamic behavior of the human p53 tumor suppressor. It was concluded the N and C termini of all four monomers did not interact and an ensemble of conformations exists that interconvert within a milisecond.

Item Type: Thesis (Bachelor's Thesis)
Degree programme: Biology
Thesis type: Bachelor's Thesis
Language: English
Date Deposited: 15 Feb 2018 07:45
Last Modified: 15 Feb 2018 07:45
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/9524

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