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Erod measurement and total cytochrome P450 contents in livers of DAB (Limanda Limanda) from the Southern North Sea

Scholtens, E. (1992) Erod measurement and total cytochrome P450 contents in livers of DAB (Limanda Limanda) from the Southern North Sea. Master's Thesis / Essay, Biology.

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Abstract

EROD activities in the microsomal- and S9 fraction and contents of total cytochrome P450 (EP450) were measured in livers of dab (Limanda limanda), caught at 15 different sites in the southern North Sea. The three types of measurements were compared with each other: a good correlation (r=O.949) was found between measurements of EROD activities in the microsomal fraction and measurements of EROD activities in the S9 fraction. The correlation between EROD values in the microsomal fraction and the EP450 concentrations in the microsomal fraction were considerably less (r=O. 580). The EROD values from stations 4, 6 and 7 were significantly higher than the values from the other stations. These stations had stratified water columns and lower temperatures of bottom water, which might explain the high EROD values. No clear pattern was observed for the EP450 data. The protein assay (Biorad-kit) was optimized.

Item Type: Thesis (Master's Thesis / Essay)
Degree programme: Biology
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 15 Feb 2018 07:47
Last Modified: 15 Feb 2018 07:47
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/9968

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