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Peroxisome heterogeneity in S. cerevisiae

Jalnapurkar B. V. (2016) Peroxisome heterogeneity in S. cerevisiae. Research Project 1 (minor thesis), UNSPECIFIED.

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Abstract

Peroxisomes contribute as a dynamic compartment in all nucleated cells. They fulfill different metabolic requirements of the cells in response to different environmental changes. Peroxisomes can adapt to changes with the modulation of the enzymes in these organelles. This can be achieved with dynamically working protein transport machinery. During peroxisome division, it gets divided into mother and daughter peroxisomes. Mother peroxisome is old in age compare to daughter peroxisomes. Hence protein content of the young and old peroxisomes is likely to be heterogeneous with respect to each other in single S.cerevisiae cell. In this study peroxisome heterogeneity is studied by analysis of protein sorting to the young and old peroxisomes. Peroxisomal proteins Pex3.GFP, Pex13.GFP, Pex25.GFP, Pex10.GFP, showed equal distribution in young and old peroxisomes whereas Pex14.GFP showed the distribution in young peroxisome. Data generated for the Pex11.GFP was insufficient to predict its behaviour. It can be concluded that peroxisome population in single S.cerevisiae cell is heterogeneous.

Item Type: Thesis (Research Project 1 (minor thesis))
Thesis type: Research Project 1 (minor thesis)
Language: English
Date Deposited: 15 Feb 2018 08:11
Last Modified: 21 Sep 2023 06:47
URI: https://fse.studenttheses.ub.rug.nl/id/eprint/13677

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