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Engineering and Expression of Sactipeptide Constructs in Lactococcus lactis

Prabha, Haritha (2018) Engineering and Expression of Sactipeptide Constructs in Lactococcus lactis. Master's Research Project 1, Biology.

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Abstract

Thurincin H, a ribosomally synthesised and post-translationally modified peptide with characteristic thiother bridges. Aim of this project is to modify six sactipeptide constructs by removing partial terminator in between structural and modification enzyme gene, and see how thnB enzyme works in Lactococcus lactis using thnA (Thurincin H structural gene) as substrate. The scope of the project is to use the sactipeptide modifications in combination with other modification systems in order to create new to nature molecules.

Item Type: Thesis (Master's Research Project 1)
Supervisor name: Kuipers, O.P.
Degree programme: Biology
Thesis type: Master's Research Project 1
Language: English
Date Deposited: 11 Oct 2018
Last Modified: 02 Nov 2018 13:58
URI: https://fse.studenttheses.ub.rug.nl/id/eprint/18716

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