The origin and applications of adipose-derived stem cells (ADSCs)

Houtsma, E. (2013) The origin and applications of adipose-derived stem cells (ADSCs). Bachelor's Thesis, Biology.

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Abstract

Recently, it has been found that mesenchymal adipose-derived stem cells (ADSCs) have many functions. ADSCs have similar properties as bone marrow mesenchymal stem cells, thereby capable of repairing and regenerating damaged tissue. ADSCs can be isolated from the stromal vascular fraction (SVF), within adipose tissue. The exact location of in vivo ADSCs in relation to their in vitro and in vivo applications is unknown. There is leading evidence that ADSCs find their origin in the perivascular environment, interacting with the vascular network. This thesis aims to look at the ADSC origin, associated with in vitro and in vivo applications. The experimental results show that mesenchymal stem cells (MSCs), including ADSCs, belong to a subset of perivascular cells, with ADSCs located in the tunica adventitia. Moreover, a pericyte subset that may be transitional between pericytes and ADSCs was found. The possible perivascular origin of ADSCs suggests that these cells interact with the vascular network, supported by various growth factors. Moreover, in vitro cultured ADSCs can be used for in vivo ADSC implantation by using scaffolds capable of vascularisation in vivo. To conclude, adipose-derived stem cells are located in a perivascular environment, thereby contributing to their in vitro and in vivo applications with regard to vascularisation. ADSC-pericyte subsets may be transitional between pericytes and ADSCs, suggesting a progenitor/progeny relationship in interaction with the vascular network. ADSCs can provide an additional way of stem cell therapy for tissue regeneration, besides bone marrow mesenchymal stem cell therapy. In the future, ADSCs may be used for in vivo applications, providing therapy for patients with obesity, neurodegenerative disease, leukaemia, ischemic diseases and cardiovascular diseases. Key words: adipose-derived stem cells (ADSCs), stromal vascular fraction (SVF), pericytes, vascularisation.

Item Type:	Thesis (Bachelor's Thesis)
Degree programme:	Biology
Thesis type:	Bachelor's Thesis
Language:	English
Date Deposited:	15 Feb 2018 07:53
Last Modified:	15 Feb 2018 07:53
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/11033

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