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Single-particle visualization of chikungunya and dengue viruses

Blijleven, J.S. (2013) Single-particle visualization of chikungunya and dengue viruses. Master's Thesis / Essay, Physics.

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Chikungunya and dengue are tropical viruses for which no vaccine is available. The research presented shows the design challenges associated with observing single-particle hemifusion kinetics of these viruses, in the long term useful for e.g. antiviral drug screening. The timescale of chikungunya fusion was determined to be in the order of a second, which is at the edge of detection for the current setup. The main challenges were keeping a constant, high temperature and ensuring the acidification to be synchronized and short. A new flow channel design was made countering the challenges. A theoretical excercise showed that the channel aspect ratio optimized for the highest average velocity at the edge of the channel is r = 1.3. Furthermore, the foundation was laid of a new software package to do automated viral fusion analysis. A single-particle study as begun in this report of chikungunya is therefore both pioneering and a promising road towards antibody screening.

Item Type: Thesis (Master's Thesis / Essay)
Degree programme: Physics
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 15 Feb 2018 07:54
Last Modified: 15 Feb 2018 07:54

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