Research Report 1 : Creating standardized vectors for fluorescent microscopy

Tiecher, C. (2014) Research Report 1 : Creating standardized vectors for fluorescent microscopy. Master's Thesis / Essay, Biology.

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Abstract

Fluorescent proteins (FPs) have been broadly employed during the last two decades in order to answer scientific questions. Since their great potential was unveiled by Prasher et al. in 1992, a lot has been done to improve the power of this tool. Its intrinsic natural fluorescence is given by the specific amino acid sequence. This makes the FPs suitable for in vivo cell imaging since they can be genetically engineered and fused to other proteins. Over the last decades several applications and set-ups have been developed to exploit these fluorescent probes. However the use of FPs to study biology has still a few drawbacks which sometimes are difficult to overcome. Often the properties of the probes are not suitable for certain experimental set ups. For instance low brightness, maturation time or half life of FPs often gives constraints in the use of live imaging studies. The creation of fusion proteins and its adaptation to a specific experimental set up is time consuming. Therefore the goal of this project is the creation of a standardized fluorescent proteins collection for Bacillus subtilis, Lactococcus lactis and Streptococcus pneumoniae. This standardized collection aims to facilitate the creation of new fusion proteins.

Item Type:	Thesis (Master's Thesis / Essay)
Degree programme:	Biology
Thesis type:	Master's Thesis / Essay
Language:	English
Date Deposited:	15 Feb 2018 07:56
Last Modified:	15 Feb 2018 07:56
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/11586

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