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Smoking women and the effect of tobacco use in the ovary

van Loo, H.A. (2015) Smoking women and the effect of tobacco use in the ovary. Master's Thesis / Essay, Biology.

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ABSTRACT INTRODUCTION: Smoke effects on fertility have been reported previously. However, how this translates to in utero exposure and the effect on fertility is not known yet. As there is an increasing amount of women including smoking in their life style, insight in the possible effects and the mechanism behind the influence is awaited. METHODS: 48 female C57BL/6 mice are whole body, main stream exposed to cigarette smoke during pregnancy. Pregnancy rates and litter sizes are studied as indicator of fertility. Histological analysis was performed by phenotyping structures in different stages of development. Immune histological analysis was performed to study potential underlying molecular mechanisms. Tissue of the mothers is used as a pilot before using tissue of the pups. RESULTS: Pregnancy rates differences between smoke and air exposed are present but not significant. Follicle classification showed a significant difference in primordial and primary follicles in ovaries of smoking pups from smoking mothers and non-smoking pups from non-smoking mothers. Immune histological analysis did not work on tissue of the pups. DISCUSSION: Pregnancy rates are influenced by the design of the study as hyperstimulation has been performed and potential cigarette smoke influences therefore could be masked. Effects in the pups on follicle counts can be studied more elaborately when smoke-air exposed pups and air-smoke exposed pups are included in the classification. This distinguishes between a direct and indirect response to cigarette smoke. CONCLUSION: This study indicates that prenatal smoke exposure influences the follicle pool. This is intuitive as the follicle pool is prenatally established. Further research is awaited to conclude how ovarian tissue reacts to cigarette smoke compounds. Both an increase in statistical power and a wider variety of techniques will provide insight in observed trend that can be explained as an adaptation effect in the pups of smoke exposed mothers.

Item Type: Thesis (Master's Thesis / Essay)
Degree programme: Biology
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 15 Feb 2018 08:09
Last Modified: 15 Feb 2018 08:09

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