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CRISPR/Cas9 a Versatile Tool in Bio-Engineering

Groen, J. (2016) CRISPR/Cas9 a Versatile Tool in Bio-Engineering. Bachelor's Thesis, Biology.

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Clustered interspaced short palindromic repeats (CRISPR) with CRISPR associated 9 (Cas9) is a new bio-engineering tool originating from bacteria. It is a powerful and versatile tool to target and bind genes of interest using RNA guidance and is subsequently able to edit or regulate genes. Cas9 can be used as a machinery that induces double-stranded breaks or it can be used to regulate genes in various ways. This review covers various aspects such as basic mechanisms of CRISPR/Cas9, CRISPR/Cas9 experimental design, off-target effects caused by CRISPR/Cas9, and catalytically inactive Cas9 with its versatile functions.

Item Type: Thesis (Bachelor's Thesis)
Degree programme: Biology
Thesis type: Bachelor's Thesis
Language: English
Date Deposited: 15 Feb 2018 08:11
Last Modified: 15 Feb 2018 08:11

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