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Characterizing the activity of Roco4 and identifying its substrates

Fikse, L. (2016) Characterizing the activity of Roco4 and identifying its substrates. Research Project 2 (major thesis), Biomedical Sciences.

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Point mutations of Leucine Rich Repeat Kinase 2 (LRRK2) are known to be pathogenic and lead to Parkinson’s disease (PD). Recent studies have shown specific Rab proteins are a substrate of LRRK2 and, through their interaction with LRRK2, influence processes such as endosomal budding. However, as LRRK2 has proven to be difficult to isolate, the homologous Roco4 from Dictyostelium discoideum is used. This study aims to determine whether various Rab proteins are substrates of Roco4 in vitro, and whether they exhibit any protein-protein interaction. Radioactive kinase assays are used to understand which Rabs are substrates. Possible substrates are then studied through radioactive kinetics assays and pull-down assays. We found that Rab1D and possibly Rab8B are substrates for Dictyostelium Roco4, and therefore may contribute to the PD phenotype.

Item Type: Thesis (Research Project 2 (major thesis))
Degree programme: Biomedical Sciences
Thesis type: Research Project 2 (major thesis)
Language: English
Date Deposited: 15 Feb 2018 08:25
Last Modified: 15 Feb 2018 08:25

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