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Metaproteomic analysis for nitrous oxide emission reduction in an Anammox and a Demon reactor

Ketelaars, R.E.A. (2016) Metaproteomic analysis for nitrous oxide emission reduction in an Anammox and a Demon reactor. Master's Thesis / Essay, Industrial Engineering and Management.

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Planctomycetes phylum are used to convert NH4+ into N2 gas. Such a reactor microbial community consists of many more kinds of bacteria interacting. Amongst these bacteria the aerobic ammonium-oxidizing bacteria (AOB) and heterotrophic denitrifiers (HDN) are known producers of nitrous oxide (N2O), a greenhouse gas 300 times more potent than CO2. Metaproteomics has been used to analyse the proteins in the anammox microbial communities with the aim of understanding these communities and reduce N2O emissions. Using 1-D SDS-PAGE and nano-liquid chromatography - tandem mass spectrometry respectively 4373 and 4647 peptide sequences, and 1820 and 2177 proteins with unique accession numbers were identified in the Olburgen and Amersfoort anammox reactors. A taxonomy analysis showed great abundancy of the anammox bacteria: Candidatus Brocadia sinica and fulgida, Jettenia, and Kuenenia. The data is in line with the expected bacteria based on reactor environmental parameters and confirmed the presence of NOB, AOB, AnAOB, HDN, the nitrogen metabolism and a few key protein. It is most probable that AOB in the form of Nitrosomonas and Nitrosospiras are responsible for any N2O emissions. The nitrifier denitrifying pathway requiring the HAO enzyme, Cytochrome C554 and chemical decomposition is the most likely source of N2O formation. The exact mechanism responsible is can only be guessed, as the data is probably not comprehensive and more detailed research is required. Further research, commercialisation, simplification and acceleration of the metaproteomic and subsequent integrated omic framework might aid the contribution of the metaproteomic research for understanding microbial communities and reducing N2O emissions.

Item Type: Thesis (Master's Thesis / Essay)
Degree programme: Industrial Engineering and Management
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 15 Feb 2018 08:27
Last Modified: 15 Feb 2018 08:27

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