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Screening of new or improved enzymes by microfluidic chips

Scheele, R.A. (2016) Screening of new or improved enzymes by microfluidic chips. Master's Thesis / Essay, Molecular Biology and Biotechnology (2016-2019).

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The functional annotation of novel protein sequences, as well as the large libraries generated through directed evolution to improve (newly found) proteins are in need for ultra-high throughput screening. Discovery and subsequent improvement of enzymes could pave the way for a broader application of enzymes in fields ranging from the chemical industry, pharmaceutics, and biotechnology to food and oil processing. Where old screening methods were time consuming and/or expensive, microfluidic chips are cheap to prepare and allow for a library size with a throughput limit of 107mutants/novel enzymes. Although the throughput limit is still one factor lower compared to fluorescent-activated cell sorting (FACS), microfluidic chips are capable of sorting water-in-oil droplets. The combination of biomimetic water-in-oil droplets with fluorescent-activated droplet sorting (FADS) makes it possible to assay a wider range of expression systems like lysed cells or excreted enzymes contained in the oil compartment. This essay looks at the development of microfluidic chips, how they work, and examples of its application. Furthermore, its limitations and what its prospects are for future research are discussed.

Item Type: Thesis (Master's Thesis / Essay)
Degree programme: Molecular Biology and Biotechnology (2016-2019)
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 15 Feb 2018 08:27
Last Modified: 15 Feb 2018 08:27

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