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Thesis: Detecting Reactive Species in Bacteria

Offens, F. (2017) Thesis: Detecting Reactive Species in Bacteria. Bachelor's Thesis, Life Science and Technology.

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Free radicals are short-lived intermediates with at least one unpaired electron which makes them highly reactive. Under normal conditations cells keep radicals at a steady-state level using several defense mechanisms. However, when there is an excessive amount of reactive species, the cell experiences oxidative stress which can cause damage to several cell compounds, such as lipids, protein and DNA and sometimes even result in cell death. Because there is still a lot unknown about free radicals and their roles in cell function and dysfunction, they are an interesting subject for research. To study these radicals, detection is neccessary, but this is challenging due to their short lifetimes, high reactivity and the low concentrations in the cell. This thesis is inteded to give an overvies of widely used methods for radical detection in bacteria. The gold standard for radical detection is by Electron Paramagnetic Resonance, however in bacteria the radical concentration often does not reach the detection limit. More commonly used methods for radical detection in bacteria are by using chemiluminescent and fluorescent probes that react with the radicals. These probes are not very specific and might react with other cell-molecules which can influence measurements. There is also an indirect method for radical detection, known as fingerprinting, which is used to assess cellular damage and therefor an indication for oxidative stress. Because there is not yet a direct and specific method for radical detection in bacteria, new methods have to be developed to get a better understanding of cell function and the role of free radicals.

Item Type: Thesis (Bachelor's Thesis)
Degree programme: Life Science and Technology
Thesis type: Bachelor's Thesis
Language: English
Date Deposited: 15 Feb 2018 08:29
Last Modified: 15 Feb 2018 08:29

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