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Fluorescence of microdroplets

Veeke, S. Van der (2016) Fluorescence of microdroplets. Master's Thesis / Essay, Applied Physics.

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Electrospray ionization (ESI) is a process where an analyte is transferred to the ionized gas phase. This technique allows fragile molecules, such as most biomolecules, to be transferred to the gas phase and to be characterized with machinery such as mass spectrometers. This process relies on the evaporation and disintegration of small microdroplets, and surprisingly isn't very well understood. This thesis presents an experimental setup that can study microdroplets during the crucial stages of the ESI evaporation process. The theory and working principles of the setup are described as well as the theory of droplet evaporation. The experimental setup relies on a quadrupole ion trap to levitate the droplets in space. The molecular distribution is tracked by making use of fluorescent molecules and a CCD-camera to capture the 2D projections of the fluorescent molecular distributions inside the droplet. The theory needed to reconstruct a 3D distribution from these 2D images is described. The final goal to capture droplets and image the molecular distribution during evaporation is not reached, however an good understanding of the working principles and operation of the experimental setup is used to design a new improved experiment. This new experiment uses an improved quadrupole trap and has several other improvements to study the properties of evaporating droplets.

Item Type: Thesis (Master's Thesis / Essay)
Degree programme: Applied Physics
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 15 Feb 2018 08:30
Last Modified: 15 Feb 2018 08:30

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