van Harmelen, Ruben (2018) Towards applied diamond magnetometry. Bachelor's Thesis, Life Science and Technology.
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Abstract
Nanodiamonds have been shown to have very unique high potential properties. Apart from many industrial applications (e.g. abrasives, catalyst), they also found their way into the medical world. Specifically, nanodiamond containing Nitrogen-Vacancy (NV) centers are very versatile promising new probes in correlative microscopy. These high-pressure high-temperature (HPHT) nanodiamonds in size tens of nanometers reveal to have an inert diamond structure, yet a highly functionalizable surface chemistry and a good internalization potential and biocompatibility. Their versatility in imaging lies in the possibility of cathodoluminescence and the virtually unlimited photo stability property which is also dependent on magnetic fluctuations up to a single electron or nucleus spin. Thus, the path to visualize on the atomic scale may have been paved. The behavior of fluorescent nanodiamond in vitro has been examined and protocols to counter aggregation in medium have been proposed. When cells do not readily take up the nanoparticles, a chemical transformation protocol has been found, which cause the cells minimum distress. In-depth research in the diamond dimensions give us insight into a flake-like diamond structure, which helps us understand biological process like endosomal escape through their relative sharp shape, as well as physical processes, like behavior in magnetic field as effect of the aspect ratio of the particle. Nanodiamonds could prove to be superior probes, replacing the currently used inferior probes like quantum dots. Also, very new imaging techniques may arise, through the possibility of visualizing magnetic fields on the nanoscale. With all this said, just de surface is scratched of the possibilities of nanodiamonds, before nanodiamonds can be declared the golden standard of imaging, much more fundamental research should be done towards in-vivo cytotoxicity and creating more efficient nanodiamonds (smaller and brighter).
| Item Type: | Thesis (Bachelor's Thesis) |
|---|---|
| Supervisor name: | Schirhagl, R. and Ong, S.Y. |
| Degree programme: | Life Science and Technology |
| Thesis type: | Bachelor's Thesis |
| Language: | English |
| Date Deposited: | 12 Jul 2018 |
| Last Modified: | 21 Aug 2019 09:38 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/17822 |
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