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Isolation of renal endothelial cells from a kidney preservation pump: a problem definition

Wees, Nienke van (2018) Isolation of renal endothelial cells from a kidney preservation pump: a problem definition. Bachelor's Thesis, Life Science and Technology.

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The research concerning the renal endothelial cells has brought a new possibility of testing the HLA types. These EC’s need to be filtered out of a perfusion pump post-transplantation. Although the current method of filtration of EC’s out of the perfusion pump fluid is sufficient, this process takes a lot of time and is labor-intensive. Rosa Lammerts MD, working in the UMCG, has provided this problem in need of a solution. The problem is in threefold; the plating of the beads is labor-intensive, time-consuming and the renal endothelial cells dedifferentiate into common endothelial cells. All of these parts contribute to the problem of slow progress for the research of the researcher. In this problem analysis, enough inside is gained to proceed to the second step of the designing process, the synthesis phase. A new method should be found that efficiently sorts the cells out of the fluid but in a faster and less labor-intensive method. The most important requirements will be taken into account, as well as the wishes. As a future perspective, also other cell types that are present in the fluid should be identified and the number of cells in this fluid counted. It is possible that these goals are inevitable to face in this designing process. The designing process can be read in the bachelor project following this thesis: “Isolation of renal endothelial cells out of a kidney preservation pump: a designing process.”

Item Type: Thesis (Bachelor's Thesis)
Supervisor name: Verkerke, G.J.
Degree programme: Life Science and Technology
Thesis type: Bachelor's Thesis
Language: English
Date Deposited: 09 Aug 2018
Last Modified: 10 Aug 2018 12:57

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