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Synthetic Transcription Factors in Filamentous Fungi

Brinkman, Lester (2019) Synthetic Transcription Factors in Filamentous Fungi. Master's Thesis / Essay, UNSPECIFIED.

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Transcription factors (TFs) in fungi regulate complex genetic processes. Synthetic transcription factors (sTFs) can be employed for biosensing, increased production of enzymes and increased secondary metabolite production. Inducible transcription factors are composed of three domains, a DNA binding domain (DNA-BD), effector binding domain (EBD) and an activating domain (AD). These domains are modular and can be exchanged. Exchange of the DNA-BD results in changing the target promoter while replacement of the EBD can change the induction condition. The activation strength can be controlled by exchange or addition of the AD. For the design of hybrid transcription factors a range of DNA-BDs and ADs are available. However only few EBDs have been characterized. In this review, general characteristics of each domain, along with several examples of hybrid TFs are discussed. Furthermore, currently described inducible transcription factors in filamentous fungi are summarized to provide a comprehensive catalogue of EBDs for the design of novel sTFs.

Item Type: Thesis (Master's Thesis / Essay)
Supervisor name: Bovenberg, R.A.L.
Thesis type: Master's Thesis / Essay
Language: English
Date Deposited: 30 Apr 2019
Last Modified: 18 Oct 2023 07:59

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