The established, and novel developments in crispr/cas mediated genetic engineering

Punt, Mats (2019) The established, and novel developments in crispr/cas mediated genetic engineering. Bachelor's Thesis, Life Science and Technology.

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Abstract

Development of a precise and high-fidelity gene editing tool has become an important goal in microbiology, and other disciplines. CRISPR/Cas systems have garnered a lot of attention for their ability to make quick and efficient DNA edits. While they have matured to a viable genetic engineering tool in many regards, a great deal of research is still necessary to increase our understanding of their functioning and range of application. Originally studied as a bacterial immune system, recruitment and utilization of engineered CRISPR/Cas systems can be performed efficiently in vitro and in vivo, and is not restricted to any organism. This review serves to give an overview of the basic functioning of CRISPR/Cas systems, their current possible applications in genetic engineering and the most important developments in CRISPR/Cas mediated genetic engineering. Also passed under review are the possible problems associated with this type of gene editing compared to other methods and the efforts in circumventing these innate problems. An attempt is also made to give insight on the safety and viability of CRISPR/Cas based genome editing platforms.

Item Type:	Thesis (Bachelor's Thesis)
Supervisor name:	Driessen, A.J.M.
Degree programme:	Life Science and Technology
Thesis type:	Bachelor's Thesis
Language:	English
Date Deposited:	14 May 2019
Last Modified:	14 May 2019 09:45
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/19471

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