Cortes Tique, Juan, J (2019) Heterologous expression and enzyme activity of chitin deacetylases from different organisms on crystalline chitin and chitosan. Master's Research Project 2, Biomolecular Sciences.
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Abstract
Heterologous expression of four chitin deacetylases (CD) from different microorganisms: Mucor rouxii (MRCD), Bacillus cereus (BC1960), Streptomyces lividans (AXE) and Arthrobacter sp (ArCE4), was attempted in order to test their enzyme activity in partially deproteinized and demineralized shrimp powder and chitosan. In addition, cultures from a waste water treatment plant were made and isolated in trace elements and chitin or chitosan as sole carbon source in the media with the aim of checking the ability to grown in such conditions. The SDS-PAGE results showed that there was no expression of MRCD, AXE and for the waste water treatment plant samples (CDCT and WACT) while the obtained protein concentration from expression cultures were 15.3 µM for BC1960 and 71.6 µM for ArCE4. No acetic acid released was detected as consequence of chitin deacetylation activity.
| Item Type: | Thesis (Master's Research Project 2) |
|---|---|
| Supervisor name: | Euverink, G.J.W. |
| Degree programme: | Biomolecular Sciences |
| Thesis type: | Master's Research Project 2 |
| Language: | English |
| Date Deposited: | 08 Jan 2020 10:26 |
| Last Modified: | 08 Jan 2020 10:26 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/21356 |
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