In Vitro Construction of the Epidermal Growth Factor Receptor Kinase Domain

Zietse, Michiel (2020) In Vitro Construction of the Epidermal Growth Factor Receptor Kinase Domain. Bachelor's Project, Pharmacy.

Preview	Text bPHAR_2020_ZietseM.pdf Download (6MB) | Preview
	Text toestemming.pdf Restricted to Registered users only Download (90kB)

Abstract

Activating mutations of the epidermal growth factor receptor (EGFR) may lead to the development of non-small cell lung cancer (NSCLC). Response to inhibition by tyrosine kinase inhibitors (TKI’s) is found to be highly variable and depends on mutation phenotype. For many rare mutations, of which clinical data is lacking, optimal treatment is yet to be determined. E. coli systems hold potential for in vitro screening of TKI inhibition of rare EGFR mutations to improve NSCLC treatment, as these systems may offer a cheap and fast screening method. During this research project, an EGFR gene construct, encoding for residue 681 to 1211, was successfully cloned in a pETM-30 vector. After expression in E. coli strains optimized for recombinant protein synthesis, the receptor construct can be used for activity screening.

Item Type:	Thesis (Bachelor's Project)
Supervisor name:	Groves, M.R. and Vilacha Madeira R Santos, J.F.
Degree programme:	Pharmacy
Thesis type:	Bachelor's Project
Language:	English
Date Deposited:	24 Jan 2020 11:03
Last Modified:	24 Jan 2020 11:03
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/21439

Actions (login required)

View Item