Talavera, Jade (2020) The role of estrogen in primary Sjögren’s syndrome salivary gland dysfunction. Master's Research Project 1, Biomedical Sciences.
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Abstract
Primary Sjögren’s syndrome (pSS) is a chronic autoimmune disease that is characterized partly by salivary gland (SG) dysfunction. Saliva is produced by acinar cells, and transported through ducts into the mouth. SG progenitor cells (SGPCs) maintain SG homeostasis, and reside potentially in the basal layer of the striated ducts (BSDs). pSS predominantly manifests in post-menopausal women, where estrogen levels are low. We aim to characterize the role of estrogen in development of pSS SG pathology. Increased ER-β expression in the BSD cells and acinar cells was detected in pSS and sicca tissues. SGPCs, cultured as organoids and treated with estrogen showed enhanced proliferation. Estrogen signaling has been suggested to promote autophagy. Expression of autophagy markers p62 and LC3B were lower in acinar cells in the vicinity of infiltrates as analyzed by scRNASeq, indicating a downregulation of autophagy. Our combined data results suggest that estrogen signaling may play a role in homeostasis of the SGPCs resident in the BSD layer of the SG. Indeed, increased ER-β expression in the BSDs of the SGs in pSS may indicate increased susceptibility for apoptosis and disruption of SG homeostasis. Acinar cell ER-β positivity by immunostaining and decrease of autophagy gene expression in acinar cells from SGs with infiltration may also suggest a protective role for estrogen signaling for acinar cells in pSS.
| Item Type: | Thesis (Master's Research Project 1) |
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| Supervisor name: | Pringle, S.A. and Coppes, R.P. |
| Degree programme: | Biomedical Sciences |
| Thesis type: | Master's Research Project 1 |
| Language: | English |
| Date Deposited: | 24 Jun 2020 11:10 |
| Last Modified: | 24 Jun 2020 11:10 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/22121 |
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