Searching for pattern recognition receptor(s) involved in the response to tick-borne encephalitis virus (TBEV) and the TBEV vaccine

Noya, M (2020) Searching for pattern recognition receptor(s) involved in the response to tick-borne encephalitis virus (TBEV) and the TBEV vaccine. Master's Research Project 1, Biomedical Sciences.

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Abstract

Tick-borne encephalitis virus (TBEV) is a major arboviral pathogen in humans, and a member of the Flaviviridae family. The virus causes tick-borne encephalitis (TBE), in which infections are mostly asymptomatic. However, TBEV can infiltrate the central nervous system and cause lesions in it, accompanied by development of neurological symptoms. Currently, a working vaccine is in use, whereas no specific treatment is available. The number of cases is slowly expanding, which enhances the need for more knowledge in this field. Previous studies showed that treatment of peripheral blood mononuclear cells (PBMCs) with TBEV and its vaccine results in an interferon (IFN) response. A gap in our knowledge includes the triggering by the virions of pattern recognition receptors (PRRs) initiating the cascade of IFN response. Therefore, the focus of this study was to search for the PRR pathway(s) involved in the response to TBEV and its vaccine. In the search for the PRR(s) involved, several assays were performed. Toll-like receptors (TLRs) and nucleotide-binding oligomerization domain 2 (NOD2) activation was assessed upon TBEV and vaccine stimulation in human reporter cells. According to this type of assay, these PRRs were not involved in the recognition of TBEV and its vaccine. The activation of TBEV vaccine-induced IFN response upon treatment with retinoic-acid-inducible gene I (RIG-I) and melanoma differentiationassociated gene 5 (MDA5) inhibitors was assessed in PBMCs. Inhibition of t

Item Type:	Thesis (Master's Research Project 1)
Supervisor name:	Huckriede, A.L.W. and Signorazzi, A.
Degree programme:	Biomedical Sciences
Thesis type:	Master's Research Project 1
Language:	English
Date Deposited:	07 Jul 2020 08:18
Last Modified:	07 Jul 2020 08:18
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/22452

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