Kuntzel, Leonie (2020) Validation of the semen analysis method used by the University Medical Center Groningen. Master's Research Project 2, Biomedical Sciences.
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Abstract
The current computer-assisted semen analysis method used by the UMCG is not always reliable and prone to technical errors, this project focusses on validating a new manual counting method to determine sperm concentration. Therefore the exact number of counted spermatozoa per field of view was counted to determine the concentration, via a calculation method that converts a number of counted spermatozoa per field into a number of spermatozoa per ml. It was determined how many fields of view per sample have to be counted in order to reach a reliable estimation of the concentration (<25% deviation of the real concentration), distinguishing between used objective (20X/40X) and location in a counting chamber. A separate part of the project was data analysis of a database containing data about semen motility parameters and fertility outcomes (fertilized oocytes/ achieved pregnancy), in an attempt to discover a potential correlation between the parameters and fertility outcome. This data analysis was performed in R studio.
| Item Type: | Thesis (Master's Research Project 2) |
|---|---|
| Supervisor name: | Hoek, A. |
| Degree programme: | Biomedical Sciences |
| Thesis type: | Master's Research Project 2 |
| Language: | English |
| Date Deposited: | 24 Aug 2020 13:38 |
| Last Modified: | 24 Aug 2020 13:38 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/23177 |
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