Sliggers, Stefan (2020) Coacervation of 10-mer polyU and RRASLRRASL-peptide. Bachelor's Thesis, Life Science and Technology.
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Abstract
Over the past two decades molecular dynamics has been used to simulate a vast number of different biomolecular systems. In this report molecular dynamics is used to replicate wet lab experiments from an experimental paper about liquid-liquid phase separation and formation of coacervates. Liquid-liquid phase separation has been hypothesized to be important in the formation of coacervates because they provide stable compartmentalization without the need of a membrane. In the experimental paper is studied the formation of coacervates through electrostatic interaction between anionic polyUs and cationic peptides. They observed a critical coacervation concentration of 250 μM, this means that coacervation was not observed when lower peptide concentrations were used. They also found a critical salt concentration of 100 mM, this means that coacervation was not observed when higher salt concentrations were used. In our simulations we found that peptides aggregate in a range from 250 μM to 1250 μM peptide concentration but that coacervation still took place at a 100 mM salt concentration.
| Item Type: | Thesis (Bachelor's Thesis) |
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| Supervisor name: | Marrink, S.J. and Tsanai, M. |
| Degree programme: | Life Science and Technology |
| Thesis type: | Bachelor's Thesis |
| Language: | English |
| Date Deposited: | 07 Dec 2020 11:18 |
| Last Modified: | 07 Dec 2020 11:18 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/23672 |
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