Hurkmans, Kim (2020) Faecalibacterium prausnitzii decreases inflammatory markers and Akkermansia muciniphila accumulates tight junction protein ZO-1 in cellular membrane of Caco-2 cells using HoxBan system. Master's Research Project 1, Biomedical Sciences.
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Abstract
The aim of the current study was to investigate whether A. muciniphila individually and together with F. prausnitzii as a bacterial consortium could be co-cultured with Caco-2 cells using the HoxBan system. First, the medium was adjusted by adding mucin from a porcine stomach to culture A. muciniphila. Next, Caco-2 cells were cultured for 18 hours with F. prausnitzii and A. muciniphila individually or in a bacterial consortium. Reduction of oxygen in the media was observed and F. prausnitzii produced a growth rim. Furthermore, a decrease in cell viability was observed when Caco-2 cells were cultured with bacteria. Moreover, F. prausnitzii showed an anti-inflammatory effect in Caco-2 cells. Interestingly, A. muciniphila showed an accumulation of tight junction protein ZO-1 in the cell membrane of Caco-2 cells while no upregulation of mRNA levels was observed. To conclude, this study showed that F. prausnitzii and A. muciniphila can be cultured together with Caco-2 cells on a HoxBan system. Therefore, opening up opportunities for other anaerobic bacteria to be co-cultured with Caco-2 cells or other host cells in an in vitro co-culture system.
| Item Type: | Thesis (Master's Research Project 1) |
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| Supervisor name: | Harmsen, H.J.M. and Fagundes Rosa, R. and Bravo Ruiseco Sanchez, G. and Faber, K.N. and Saeed, A. |
| Degree programme: | Biomedical Sciences |
| Thesis type: | Master's Research Project 1 |
| Language: | English |
| Date Deposited: | 04 Jan 2021 11:19 |
| Last Modified: | 04 Jan 2021 11:19 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/23770 |
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