Increasing Gene knock-in efficiency and specificity of CRISPR/Cas9 for future applications

Zijlstra, Johan (2021) Increasing Gene knock-in efficiency and specificity of CRISPR/Cas9 for future applications. Bachelor's Thesis, Biology.

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Abstract

The CRISPR-Cas9 gene editing tool has seen widespread use since its development a decade ago. The accurate and simple targeting of the Cas9 nuclease to nearly anywhere in the genome allows for a wide range of gene editing purposes. It currently is reliant on host cell DNA repair pathways to repair the generated double stranded break via either NHEJ or HDR. A reliance that is hindering the potential for further experimental and therapeutic purposes. This review aims to identify and discuss recent improvements that may improve efficiency of CRISPR-Cas9 mediated knock-ins. Several groups have attempted and achieved more efficient ways to deliver the CRISPR-Cas9 system, increased the rate of HDR DNA repair, inhibit NHEJ repair and even develop mostly host cell independent gene editing tools based on Cas9. Most of the discussed improvements require further development but do have the potential to widen the range of applications even further.

Item Type:	Thesis (Bachelor's Thesis)
Supervisor name:	Foijer, F.
Degree programme:	Biology
Thesis type:	Bachelor's Thesis
Language:	English
Date Deposited:	11 Feb 2021 16:07
Last Modified:	11 Feb 2021 16:07
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/23955

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