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Isotope labelling-based quantitative proteomics: A comparison of labelling methods

Ahmadyar, Philip (2021) Isotope labelling-based quantitative proteomics: A comparison of labelling methods. Bachelor's Thesis, Pharmacy.

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In the pursuit of identifying and quantifying biomarkers, quantitative proteomics has become a very important tool. These biomarkers are differentially expressed or modified in the onset or progression of a disease and provide important information for the diagnosis, prognosis and potential therapeutic targets for treatment. Relative quantitative proteomics aims to reveal which proteins are differentially expressed between samples. Bottom-up proteomics is the identification of proteins by analysing peptides generated from the proteolysis of proteins whereas top-down proteomics analyse intact proteins directly. Bottom-up proteomics is more widely used due to peptides being more easily ionised and fragmented. This review focused on comparing the strengths and limits of the following isotopic and isobaric labelling methods: Stable Isotope Labelling by amino acids in cell Culture (SILAC), dimethyl labelling, Cleavable Isotope Coded Affinity Tag (cICAT), Isotope-Coded Labelling (ICPL), 16O/18O labelling, Isobaric tag for relative and absolute quantitation (iTRAQ) and Tandem Mass Tags (TMT). Moreover, the discussion was devoted to discussing which methods are most suited for the different kind of samples. This literature study concludes that all the different labelling methods are suited for different kinds of samples due to their strengths and drawbacks and that there is no one best method for all samples.

Item Type: Thesis (Bachelor's Thesis)
Supervisor name: Tian, X. and Bischoff, R.P.H.
Degree programme: Pharmacy
Thesis type: Bachelor's Thesis
Language: English
Date Deposited: 22 Jun 2021 14:36
Last Modified: 24 Jun 2021 08:58

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