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Uncovering Strategies to Ensure Maximum Viability and Stability during Freeze-drying and storage of Faecalibacterium prausnitzii - a potential Next Generation Probiotic.

Ruiter, Ilse de (2021) Uncovering Strategies to Ensure Maximum Viability and Stability during Freeze-drying and storage of Faecalibacterium prausnitzii - a potential Next Generation Probiotic. Research Project 1, Biomedical Sciences.

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Abstract

Due to Faecalibacterium prausnitzii’s anti-inflammatory effects, butyrate production and lowered levels in dysbiosis-related disorders, it is considered an interesting candidate for Next Generation Probiotic development. The aim of this investigation was to discover different elements to protect strict anaerobic bacterium F. prausnitzii during the manufacturing process, specifically during freeze-drying. Using literature, cryoprotectants as well as oxygen-reducing storage options were investigated, for the ultimate purpose of maintaining high viability and stability during storage and ingestion. Furthermore, additional protective elements were studied including encapsulation techniques and companies with novel capsule technologies were reviewed. A summarized protective mixture was formulated for F. prausnitzii. Additionally, a laboratory experiment was performed to test a cryoprotective solution containing riboflavin, inulin and cysteine (M. T. Khan et al., 2014), while also testing whether extracellular polymeric substances (EPS) produced by F. prausnitzii offered extra protection during freeze-drying. In conclusion, this research shows that optimizing the freeze drying process is rewarding in terms of viability and yield of the probiotic, so far small steps have been taken, but it’s the foundation for more research to develop F. prausnitzii as a potential Next Generation Probiotic in terms of manufacturing, freeze-drying and storage.

Item Type: Thesis (Research Project 1)
Supervisor name: Harmsen, H.J.M.
Degree programme: Biomedical Sciences
Thesis type: Research Project 1
Language: English
Date Deposited: 14 Jul 2021 11:45
Last Modified: 14 Jul 2021 11:45
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/25220

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