Kassai, Sonia (2021) The use of oligonucleotide hybridization probes to distinguish bacterial species in electron microscopy. Research Project 1, Biomedical Sciences.
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Abstract
For centuries now, microscopy has been a crucial part of the study of many biological phenomena. As scientific research is moving forward faster than ever, new imaging technologies are needed to aid in breakthroughs as well as their real-life applications. Some major breakthroughs in microscopy include electron microscopy and fluorescent microscopy, which can show us cellular ultrastructure and localization respectively. However, individually these techniques can tell us only part of a complete picture. In order to get a better overview, these two techniques have been joined to form correlated light and electron microscopy (CLEM). Through the use of CLEM, more complete and high-resolution images can be obtained. This technique has been on the rise in fields of neurobiology, but its applications for microbiology have not yet been explored. Many studies focus on understanding the gut microbiome, and specifically an important bacterium Faecalibacterium prausnitzii (F. prausnitzii). CLEM could be a useful tool in understanding its ecology. In this paper, we aimed at developing a prospective method for preparing bacterial samples for visualization and identification using CLEM. More specifically, a technique for the visualization and identification of F. prausnitzii will be discussed.
| Item Type: | Thesis (Research Project 1) |
|---|---|
| Supervisor name: | Harmsen, H.J.M. and Bravo Ruiseco Sanchez, G. |
| Degree programme: | Biomedical Sciences |
| Thesis type: | Research Project 1 |
| Language: | English |
| Date Deposited: | 19 Jul 2021 13:13 |
| Last Modified: | 19 Jul 2021 13:13 |
| URI: | http://fse.studenttheses.ub.rug.nl/id/eprint/25274 |
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