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Changes In The Frequency Of B-Cell Subsets In Anca-Associated Vasculitis And Pemphigus Vulgaris; A Cross-Sectional Study

Nacy, Diana (2021) Changes In The Frequency Of B-Cell Subsets In Anca-Associated Vasculitis And Pemphigus Vulgaris; A Cross-Sectional Study. Research Project 1, Biomedical Sciences.

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Abstract

Introduction: autoimmune diseases have a high prevalence in the population and can be the result of various disturbances in immunological tolerance. Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) and Pemphigus Vulgaris are two B-cell mediated autoimmune diseases. The mechanisms that trigger these diseases are not fully understood yet. However, changes in function, phenotype, and frequency of B-cells could contribute to the pathogenesis of these diseases. This study aims to investigate which B cell subsets are associated with active AAV and Pemphigus and whether these subsets differ from the healthy controls (HCs). Methods: Fresh blood samples from 3 active GPA patients, 5 active Pemphigus patients, and 5 HC were stained using a multiparameter flow cytometry panel that encompasses the surface markers CD19, CD27, CD28, CD24, and IgD. Data were analyzed using a t-distributed Stochastic Neighbor Embedding (t-SNE) analytical tool to visualize several B-cell clusters based on their expression markers. Five Clusters that had significant differences in the distribution between the patients and HCs were further analyzed using traditional gating methods based on the expression level of their markers. For this purpose, data from 9 GPA- and 15 Pemphigus patients and 15 HCs were used. The percentage of B cells in each cluster was statistically analyzed to determine if the groups had significant differences compared to each other. Results: the t-SNE analysis

Item Type: Thesis (Research Project 1)
Supervisor name: Heeringa, P. and Bijma, T.
Degree programme: Biomedical Sciences
Thesis type: Research Project 1
Language: English
Date Deposited: 12 Aug 2021 06:07
Last Modified: 12 Aug 2021 06:07
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/25576

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