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Changes In The Frequency Of B-Cell Subsets In ANCA-Associated Vasculitis and Pemphigus Vulgaris; A Cross-Sectional Study

Nacy, Diana (2021) Changes In The Frequency Of B-Cell Subsets In ANCA-Associated Vasculitis and Pemphigus Vulgaris; A Cross-Sectional Study. Research Project 1, Biomedical Sciences.

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Abstract

Antineutrophil Cytoplasmic Antibody (ANCA)-associated vasculitis (AAV) and Pemphigus Vulgaris are two B-cell mediated autoimmune diseases. The mechanisms that trigger these diseases are not fully understood yet. However, changes in function, phenotype, and frequency of B-cells could contribute to the pathogenesis of these diseases. This study aims to investigate which B cell subsets are associated with active AAV and Pemphigus and whether these subsets differ from the healthy controls (HCs). Data were analyzed using a t-distributed Stochastic Neighbor Embedding (t-SNE) analytical tool to visualize several B-cell clusters based on their expression markers. Five Clusters with significant differences in the distribution were further analyzed using traditional gating methods. The t-SNE analysis revealed that there were significant differences in the levels of marker expression in various clusters. Naive B cells were significantly decreased in patient groups compared to the HCs. Only GPA patients had an increased frequency in transitional compared to HCs, while Pemphigus showed decreased frequencies. Double switched memory B cells were greatly increased in 2 out of the 5 pemphigus patients. Switched memory B cells were decreased in both GPA and Pemphigus compared to

Item Type: Thesis (Research Project 1)
Supervisor name: Heeringa, P. and Bijma, T.
Degree programme: Biomedical Sciences
Thesis type: Research Project 1
Language: English
Date Deposited: 23 Aug 2021 12:53
Last Modified: 23 Aug 2021 12:53
URI: http://fse.studenttheses.ub.rug.nl/id/eprint/25643

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