Benzenetriol and derivatives: Potential activity against citrus canker

Atlason, Úlfur Ágúst (2021) Benzenetriol and derivatives: Potential activity against citrus canker. Master's Research Project 1, Biomolecular Sciences.

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Abstract

Citrus canker is a plant disease which plagues citrus production worldwide, caused by the bacterium Xanthomonas citri sp. citri. Anti-microbial sprays, traditionally containing copper compounds, are used to limit the spread of the bacteria. Due to the adverse environmental effect of copper use, alternatives have been sought. Previous research into organic compounds derived from benzene-1,2,4-triol (BTO) revealed that BTO itself also had anti-bacterial activity. BTO is a reactive compound, forming dimers when exposed to air. Thus it was not known if it was BTO or the dimerization derivatives which contributed this activity. The results of this project show that it is BTO itself which provides high antibacterial activity, with the dimer showing lesser activity. Furthermore, the activity of BTO decreases over time in solution, while the activity of dimers does not. The effect of BTO and dimers on membrane permeability, metabolism and intracellular reactive oxygen species generation was investigated. No such effect was observed, with a possible exception of one dimer potentially reducing intracellular ATP concentration. The complicated background fluorescence of the compounds in media made it difficult to interpret experiments involving fluorescence reporters.

Item Type:	Thesis (Master's Research Project 1)
Supervisor name:	Scheffers, D.J. and Bonci Cavalca, L.
Degree programme:	Biomolecular Sciences
Thesis type:	Master's Research Project 1
Language:	English
Date Deposited:	19 Nov 2021 14:55
Last Modified:	19 Nov 2021 14:55
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/26302

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