Cywinska, Aleksandra Anna (2021) THE INFLUENCE OF MICROENVIRONMENT ON NEURAL PROGENITOR CELLS IN HEALTHY AND DISEASED STATES. Research Project 2 (major thesis), Behavioural and Cognitive Neurosciences.
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Abstract
Microenvironment plays a big role in modulating cellular behavior. Especially in the case of stem cells, proliferation and fate determination is largely influenced by extracellular cues. Moreover, in neurodegenerative diseases such as Alzheimer’s Disease, the neurodegeneration often occurs alongside a pre-determined anatomical order – possibly due to different extracellular matrix (ECM) features in different brain regions. Here, we focused on investigating the influence of scaffold composition, ultrastructure and stiffness on the neuronal progenitor cells (NPCs) morphology, proliferation and differentiation. To investigate that, we compared the influence of ECM obtained from different brain regions on NPCs derived from induced pluripotent stem cells from AD patients and controls. To assess the cellular interaction with the scaffold, NPCs were seeded on 3D porous nanostructures of two different scales. Separately, we also tested hydrogels of varying stiffnesses and we worked on optimizing the procedure for region-specific brain decellularization. Our preliminary data show that NPCs seeded in stiffer gels display higher proliferation while the morphology of the cells is more elongated in softer gels. The first seeding of NPCs on the scaffolds showed that they can interact with it and enter the inner structures, provided the pores are large enough. Our results will help clarify the mechanisms by which the microenvironment modulates progenitor cell niche in neurodegeneration.
| Item Type: | Thesis (Research Project 2 (major thesis)) |
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| Supervisor name: | Dolga, A.M. and Trombetta Lima, M. |
| Degree programme: | Behavioural and Cognitive Neurosciences |
| Thesis type: | Research Project 2 (major thesis) |
| Language: | English |
| Date Deposited: | 06 Dec 2021 08:49 |
| Last Modified: | 15 Feb 2024 14:36 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/26344 |
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