Javascript must be enabled for the correct page display

Culturing Dupuytren Fibroblasts in Hypoxic Conditions Presents Different Levels of α-SMA, Collagen Type I and Collagen Modifying Enzymes

Milutinovic, Masa (2022) Culturing Dupuytren Fibroblasts in Hypoxic Conditions Presents Different Levels of α-SMA, Collagen Type I and Collagen Modifying Enzymes. Bachelor's Research Project (period 2a), Biology.

[img]
Preview
 Text
[bBIO ]_[2022]_[Masa Milutinovic].pdf
Download (711kB) | Preview
[img] Text
toestemming.pdf
Restricted to Registered users only
Download (162kB)

Abstract

Dupuytren’s disease (DD) is a benign condition characterized by excess deposition of collagen type I in the extracellular matrix in the palmar fascia and is associated with high presence of myofibroblasts. Transformation of fibroblasts to myofibroblasts is regulated by TGFβ. Myofibroblasts are subject to hypoxic conditions in vivo and therefore need to adapt, with the help of the HIF1-α transcriptional regulator. Previously, myofibroblasts cells have been cultured and studied under 20% oxygen (normoxia), whereas physiological conditions present 3-5% oxygen concentration (hypoxia). Here we set out to study and compare the varying gene expression of ACTA2, COL1A1/2, and collagen modifying genes in myofibroblasts in normoxic and hypoxic conditions with and without TGFβ presence. We show that gene expression varies based on the oxygen concentrations and TGFβ presence, as well as describing the differences observed when comparing DD cells to the controls under the different conditions. Taken together, we conclude that culturing under hypoxic conditions provides a more representative environment for Dupuytren cells, therefore leading to more accurate conclusions in future studies.

Item Type: Thesis (Bachelor's Research Project (period 2a))
Supervisor name: Bank, R.A. and Borghuis, T.
Degree programme: Biology
Thesis type: Bachelor's Research Project (period 2a)
Language: English
Date Deposited: 08 Apr 2022 09:10
Last Modified: 08 Apr 2022 09:10
URI: https://fse.studenttheses.ub.rug.nl/id/eprint/26830

Actions (login required)

View Item View Item