Teisman, Joeri (2022) The effect of LPS on intra- and extracellular Alkaline phosphatase levels and their relation to hepatic fibrosis. Bachelor's Project, Pharmacy.
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Abstract
To study the influence of different levels of LPS on the amounts of alkaline phosphatase and the relation of alkaline phosphatase to liver fibrosis. This is done with an alkaline phosphatase activity assay on the conversion of pNPP to pNP. A qPCR is done on a house keeping gene GAPDH to standardize the measurements and a qPCR is done on the RNA encoding for the Alkaline phosphatase and the TLR4 receptor. With an immunohistochemical experiment the increased amounts of IGF-II, collagen I, fibronectin and AP are visualized and analyzed. The activity assay showed no significant increase in Alkaline Phosphatase activity compared to the negative control. The qPCR of alkaline phosphatase and the TLR4 receptor did not give representable results. The immunohistochemical experiment showed increased levels of IGF-II, collagen I, fibronectin, and AP in specific areas of the liver tissue.
| Item Type: | Thesis (Bachelor's Project) |
|---|---|
| Supervisor name: | Poelstra, K. and Duin, M. |
| Degree programme: | Pharmacy |
| Thesis type: | Bachelor's Project |
| Language: | English |
| Date Deposited: | 11 Apr 2022 12:51 |
| Last Modified: | 11 Apr 2022 12:51 |
| URI: | https://fse.studenttheses.ub.rug.nl/id/eprint/26861 |
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