In vitro vs. in vivo: Is it time to reduce dependence on the latter?

Sarkar, Sucharita (2022) In vitro vs. in vivo: Is it time to reduce dependence on the latter? Bachelor's Thesis, Life Science and Technology.

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Abstract

Biological ‘wet lab’ research can broadly be classified into in vivo, ex vivo and in vitro categories. So far, in vivo experimentation involving animals has been essential before a vaccine or drug candidate could be moved to a clinical trial phase but because of the cost, time, resource and ethical concerns involved with those in vivo models, not to mention the chance that clinical trials do not always have the same success rate has made in vivo experiments cumbersome. Hence, there is an urgent need to look towards in vitro models and measure their reliability. The research questions explored in this thesis are : (1) to what extent can in vitro studies substitute in vivo studies and, (2) can data from in vitro studies be credible enough to reduce the dependence on in vivo studies? The two main in vitro models that are in focus are the peripheral blood mononuclear cell (PBMC) model and the modular immune in vitro construct (MIMIC) system. The PBMC model involves separating PBMCs from whole blood via density gradient centrifugation or leukapheresis, subsequently stimulating those cells with a vaccine, adjuvant or drug candidate of interest and performing functional assays on them. The MIMIC system is divided into four parts that can broadly be explained as the collection of white blood cells, the Peripheral Tissue Equivalent (PTE) module that mimics innate immune response, the Lymphoid Tissue Equivalent (LTE) module that mimics adaptive immune response (continued)

Item Type:	Thesis (Bachelor's Thesis)
Supervisor name:	Huckriede, A.L.W. and Gong, S.
Degree programme:	Life Science and Technology
Thesis type:	Bachelor's Thesis
Language:	English
Date Deposited:	19 Apr 2022 09:58
Last Modified:	19 Apr 2022 09:58
URI:	https://fse.studenttheses.ub.rug.nl/id/eprint/26937

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